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AFLP and SRAP markers linked to the mj gene for root-knot nematode resistance in cucumber Scientia Agricola
Devran,Zübeyir; Firat,Ahmet Fikret; Tör,Mahmut; Mutlu,Nedim; Elekçioğlu,Ibrahim Halil.
Root-knot nematodes (Meloidogyne spp.) are an important worldwide pest of cucumber (Cucumis sativus L.). Molecular markers linked to the Javanese root-knot nematode (M. javanica) resistance gene mj in cucumber may aid marker assisted selection. One-hundred AFLP (EcoRI-MseI) and 112 SRAP were used to screen resistant and susceptible parents for polymorphisms to develop molecular markers linked to the mj gene. Of the 100 AFLP primers, 92 produced bands and two yielded candidate markers (E-ATT/M-CAA and E-AAC/M-CTG). These two bands were cut off from polyacrylamide gel, cloned and sequenced. Primers designed from the sequences did not yield polymorphic bands between the parents. In addition, the sequences did not contain any restriction site or indel to be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Meloidogyne javanica; Resistance; Molecular breeding.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000100017
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The genetic dissection of quantitative traits in crops Electron. J. Biotechnol.
Semagn,Kassa; Bjørnstad,Åsmund; Xu,Yunbi.
Most traits of interest in plant breeding show quantitative inheritance, which complicate the breeding process since phenotypic performances only partially reflects the genetic values of individuals. The genetic variation of a quantitative trait is assumed to be controlled by the collective effects of quantitative trait loci (QTLs), epistasis (interaction between QTLs), the environment, and interaction between QTL and environment. Exploiting molecular markers in breeding involve finding a subset of markers associated with one or more QTLs that regulate the expression of complex traits. Many QTL mapping studies conducted in the last two decades identified QTLs that generally explained a significant proportion of the phenotypic variance, and therefore, gave...
Tipo: Journal article Palavras-chave: Association mapping; Linkage disequilibrium; Markers assisted selection; Molecular breeding; Molecular markers; Quantitative trait; QTL mapping; QTL analysis.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500016
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